johnnyphung / biology / 05:_DNA / 5.08:_DNA_Sequencing_by_the_Dideoxy__Method

5.08: DNA Sequencing by the Dideoxy Method

Diagram showing the conversion of ATP to methylthioadenosine. ATP loses pyrophosphate to form DMAPP, which then becomes MTA. Chemical structures and bonds are depicted throughout.
Figure 5.8.2: Sample DNA Sequencing

The DNA to be sequenced is prepared as a single strand. This template DNA is supplied with

Colorful DNA sequencing chromatogram with four rows of peaks in red, yellow, black, and gray, each representing different nucleotide bases.
Figure 5.8.3 example of a DNA sequence (455 nucleotides of the lysU gene of E. coli) courtesy of Pharmacia Biotech Inc., Piscataway, NJ